| Expression pattern: |
UP |
| Associated gene: |
SUCLG2, AGO2 |
| Associated microRNA: |
miR-338-5p |
| Biological function: |
promotes proliferation; inhibits apoptosis; maintains mitochondrial function and TCA cycle to support tumor progression |
| Molecular mechanism: |
ceRNA mechanism: circPSD3 sponges miR-338-5p to upregulate SUCLG2, sustaining the TCA cycle and mitochondrial function |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); mitochondrial function (promotes); oxidative phosphorylation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; ISH (In Situ Hybridization); RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; EdU Staining; CCK8; Annexin V/PI Flow Cytometry; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
circPSD3 expression level was positively correlated with larger tumor size; tumors with lymph node metastasis exhibited a higher expression level of circPSD3 |
| Description: |
circPSD3 is upregulated in thyroid carcinoma/PTC and correlates with tumor size and lymph node metastasis. It promotes tumor cell proliferation and suppresses mitochondria-mediated apoptosis by sponging miR-338-5p to upregulate SUCLG2, sustaining the TCA cycle and mitochondrial respiration. |
| Confidence score: |
0.853 |