| Expression pattern: |
DN |
| Associated gene: |
p53, Bax, Bcl-2 |
| Associated microRNA: |
- |
| Biological function: |
Promotes apoptosis and inhibits cell activity/proliferation in colorectal cancer cells. |
| Molecular mechanism: |
circZNF609 overexpression upregulates p53 and shifts Bax/Bcl-2 to induce apoptosis. |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
divergent primers PCR; RT-qPCR; Sanger Sequencing; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation; ROC Analysis |
| Clinical significance: |
Down-regulated in serum of patients with colorectal cancer and showed diagnostic value by ROC analysis (AUC 0.767). |
| Description: |
circZNF609 is down-regulated in colorectal cancer tissues and patient serum. Its overexpression in HCT116 cells inhibits cell activity and promotes apoptosis, accompanied by increased p53 and Bax and decreased Bcl-2, suggesting a pro-apoptotic role via p53 upregulation. Serum circZNF609 shows potential diagnostic value based on ROC analysis. |
| Confidence score: |
0.7353 |