| Expression pattern: |
DN |
| Associated gene: |
TOB1 |
| Associated microRNA: |
miR-3615 |
| Biological function: |
inhibits proliferation; promotes autophagy; inhibits malignant phenotype |
| Molecular mechanism: |
exosomal circRNA acts as a miRNA sponge binding miR-3615; TOB1 positively regulates hsa_circ_0008719; hsa_circ_0008719 negatively regulates miR-3615 |
| Biological pathway or process: |
autophagy (promotes); proliferation (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Western Blot; Clinical Sample Validation |
| Clinical significance: |
hsa_circ_0008719 was significantly downregulated in gastric cancer tissues and low expression was significantly associated with moderate to poor differentiation |
| Description: |
In gastric cancer, hsa_circ_0008719 is down-regulated in patient tumor tissues but is upregulated by TOB1 in gastric cancer cells/exosomes. It localizes mainly in the cytoplasm and acts as a sponge for miR-3615; exosomal delivery of hsa_circ_0008719 suppresses proliferation and enhances autophagy in recipient gastric cancer cells. |
| Confidence score: |
0.8035 |