| Expression pattern: |
DN |
| Associated gene: |
PTPN11 |
| Associated microRNA: |
miR-661 |
| Biological function: |
Inhibits cell viability and colony formation; induces apoptosis; causes G1 accumulation (cell-cycle arrest). |
| Molecular mechanism: |
Proposed ceRNA mechanism: hsa-circ-0000221 inhibits (sponges) miR-661, leading to increased PTPN11 mRNA; associated with caspase-dependent, mitochondrial-dependent apoptosis. |
| Biological pathway or process: |
apoptosis (promotes); cell cycle (inhibits); proliferation (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Sanger Sequencing; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot |
| Clinical significance: |
Depletion of serum hsa-circ-0000221 is proposed as a stable serum biomarker for HCC diagnosis. |
| Description: |
In HCC, hsa-circ-0000221 (VIM-derived) is down-regulated in patient serum and functions as a tumor suppressor in vitro. Its overexpression in HepG2/SNU449 inhibits miR-661 and increases PTPN11 mRNA, reducing viability/colony formation and inducing G1 accumulation and caspase/mitochondria-associated apoptosis. |
| Confidence score: |
0.6536 |