| Expression pattern: |
DS |
| Associated gene: |
IGF2BP1, NACC1, hnRNPK |
| Associated microRNA: |
- |
| Biological function: |
suppresses proliferation, colony formation, stemness and tumorigenesis in colorectal cancer |
| Molecular mechanism: |
circNRIP1 binds IGF2BP1 (KH_1/2 domain), inhibits IGF2BP1 m6A reader activity, decreases NACC1 mRNA stability; circNRIP1 is secreted via hnRNPK-exosome pathway |
| Biological pathway or process: |
proliferation (inhibits); stemness (inhibits); mRNA stability (inhibits); m6A modification (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
S
M
|
| Validation methods: |
RT-qPCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); MeRIP / MeRIP-seq; Transfection; CCK8; Colony Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Microarray; RNA-seq; Clinical Sample Validation; ROC Analysis; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
blood exosomal circNRIP1 could be an early diagnostic biomarker for CRC |
| Description: |
In colorectal cancer, circNRIP1 is downregulated in tumor tissues but elevated in blood exosomes and can serve as an early diagnostic biomarker. Functionally, circNRIP1 suppresses CRC proliferation/stemness and xenograft tumor growth by binding IGF2BP1 (KH1/2), inhibiting its m6A reader activity and reducing NACC1 mRNA stability; circNRIP1 export may involve the hnRNPK-exosome pathway. |
| Confidence score: |
0.8898 |