| Expression pattern: |
UP |
| Associated gene: |
TXNIP, NLRP3 |
| Associated microRNA: |
miR-532-3p |
| Biological function: |
promotes pyroptosis; promotes inflammation; exacerbates NAFLD progression |
| Molecular mechanism: |
circSOD2 sponges miR-532-3p to relieve repression of TXNIP, activating the TXNIP/NLRP3 inflammasome signaling pathway to promote pyroptosis |
| Biological pathway or process: |
pyroptosis (promotes); inflammation (promotes); NF-kappaB (promotes) |
| Detected method: |
Q
B
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Northern Blot; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; circRIP; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Western Blot; ELISA; Flow Cytometry（Non-apoptosis/cycle）; JC-1 staining; Oil Red O Staining; IHC (Immunohistochemistry); TUNEL; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circSOD2 is upregulated in NAFLD and mainly localized in the cytoplasm. It functions as a ceRNA to sponge miR-532-3p, thereby de-repressing TXNIP and activating the TXNIP/NLRP3 inflammasome cascade, which promotes inflammatory pyroptosis in hepatocytes and worsens NAFLD phenotypes in vivo. |
| Confidence score: |
0.8661 |