| Expression pattern: |
UP |
| Associated gene: |
TNS3, EIF4A3, AGO2 |
| Associated microRNA: |
miR-381-3p |
| Biological function: |
promotes ESCC cell proliferation, migration, invasion and in vivo tumor growth |
| Molecular mechanism: |
EIF4A3 promotes biogenesis of hsa_circ_0001165; hsa_circ_0001165 sponges miR-381-3p and upregulates TNS3 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0001165 expression is positively associated with patients' TNM staging; potential biomarker and therapeutic target for ESCC |
| Description: |
hsa_circ_0001165 is up-regulated in ESCC tissues and cell lines and promotes proliferation, migration, invasion, and xenograft tumor growth. Mechanistically, EIF4A3 enhances hsa_circ_0001165 biogenesis, and hsa_circ_0001165 functions as a ceRNA by sponging miR-381-3p to increase TNS3 expression. |
| Confidence score: |
0.8771 |