| Expression pattern: |
UP |
| Associated gene: |
EIF4A3, CDC42 |
| Associated microRNA: |
- |
| Biological function: |
Inhibits irradiated hepatic stellate cell activation by inhibiting proliferation, promoting apoptosis, reducing proinflammatory cytokine secretion, and decreasing profibrotic markers; suppresses radiation-induced liver fibrosis. |
| Molecular mechanism: |
circ96498 binds EIF4A3 and impedes its irradiation-induced nuclear translocation, reducing EIF4A3-mediated CDC42 mRNA stability and CDC42 expression, thereby suppressing NF-kappaB and JNK/Smad2 pathway activation. |
| Biological pathway or process: |
NF-kappaB (inhibits); TGF-beta/SMAD (inhibits); MAPK (inhibits); proliferation (inhibits); apoptosis (promotes); inflammation (inhibits); fibrosis (inhibits); mRNA stability (inhibits) |
| Detected method: |
Q
H
|
| Validation methods: |
FISH / smFISH; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot; IF (Immunofluorescence); RNA Pull-Down; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining |
| Clinical significance: |
circ96498 could serve as a rapid and noninvasive biomarker for early diagnosis and a potential therapeutic target for RILF. |
| Description: |
hsa_circ_0096498 (circ96498), derived from RSF1, is up-regulated in irradiated hepatic stellate cells in radiation-induced liver injury/fibrosis. It binds EIF4A3 to prevent its nuclear translocation, lowering CDC42 mRNA stability and CDC42 expression, thereby inhibiting NF-kappaB and JNK/Smad2 signaling and suppressing inflammatory/fibrotic activation of HSCs and RILF progression. |
| Confidence score: |
0.8416 |