circRNA basic information
circBase ID: hsa_circ_0016662
Name: hsa_circ_ENAH
Synonym: -
Host Gene: ENAH
Genomic location(hg19): chr1:225742607-225755116:-
Genomic location(hg38): chr1:225554905-225567414:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tumor tissues; adjacent normal liver tissues

Cell lines:

Huh7; HCCLM3; HepG2; MHCC-97 H; MHCC-97 L; SNU-449; L02; 293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: YWHAZ
Associated microRNA: hsa-miR-22-3p
Biological function: promotes HCC proliferation, migration and metastasis; promotes autophagy; promotes HCC growth in vivo
Molecular mechanism: ceRNA/miRNA sponge: hsa_circ_0016662 sponges hsa-miR-22-3p to regulate YWHAZ, involving autophagy in HCC progression
Biological pathway or process:

autophagy (promotes); proliferation (promotes); migration (promotes); metastasis (promotes); ceRNA regulation (promotes)

Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis; Clinical Sample Validation; Cohort Study

Clinical significance:

prognostic biomarker in HCC; upregulated in HCC tissues

Description:

hsa_circ_0016662 is upregulated in HCC tissues and cell lines and functions as an oncogenic circRNA. It promotes HCC proliferation, migration and metastasis by sponging hsa-miR-22-3p to regulate YWHAZ, with autophagy contributing to the malignant phenotype.

Confidence score:

0.7738

Other information
Title:

Hsa_circ_0016662 acts as a prognostic biomarker and promotes the progression of hepatocellular carcinoma via autophagy regulation.

Journal: Journal of translational medicine
Published: 2025
PubMed ID: 40597269
Study type:

combined biological and clinical study

Data availability: GSE155949; TCGA-LIHC; E_TABM_36; GSE144269; GSE14520; GSE54236
Code availability: -