| Expression pattern: |
UP |
| Associated gene: |
STAT1, Ranbp2, SLC7A11, GPX4, IFNgamma |
| Associated microRNA: |
- |
| Biological function: |
Promotes melanoma cell proliferation, limits ICB therapy efficacy, and restricts IFNgamma-induced immunogenic ferroptosis. |
| Molecular mechanism: |
circPIAS1 encodes a 108-aa peptide (circPIAS1-108aa) that recruits Ranbp2 to enhance STAT1 SUMOylation, thereby inhibiting STAT1 phosphorylation and reactivating the SLC7A11/GPX4 axis to suppress IFNgamma-induced immunogenic ferroptosis. |
| Biological pathway or process: |
ferroptosis (inhibits); apoptosis (other); proliferation (promotes); immune regulation (inhibits); other pathway/process (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; FISH / smFISH; Transfection; CCK8; EdU Staining; Colony Formation Assay; Luciferase Reporter Assay; Western Blot; RIP (RNA Immunoprecipitation); Co-IP; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
circPIAS1 is increased in melanoma tumor tissues versus adjacent/normal tissues and is a potential therapeutic target to enhance PD-1 inhibitor efficacy. |
| Description: |
circPIAS1 is upregulated in melanoma tumors and promotes tumor cell proliferation while limiting PD-1 blockade efficacy. It encodes a 108-aa peptide that recruits Ranbp2 to increase STAT1 SUMOylation, suppress STAT1 phosphorylation, reactivate SLC7A11/GPX4, and inhibit IFNgamma-induced immunogenic ferroptosis. |
| Confidence score: |
0.8354 |