circRNA basic information
circBase ID: hsa_circ_0006174
Name: -
Synonym: circ_0006174 / hsa_circRNA_104852
Host Gene: -
Genomic location(hg19): chr9:110064315-110068928:+
Genomic location(hg38): chr9:107302034-107306647:+
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; DOX-resistant CRC tissues; DOX-sensitive tissues; normal tissues; tumor tissues; normal cancerous peripheral tissues
Cell lines:

LoVo; HCT116; LoVo/DOX; HCT116/DOX
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CCND2, P-gp, GSTpi
Associated microRNA: miR-1205
Biological function: Promotes doxorubicin chemoresistance, cell proliferation, migration, and invasion in colorectal cancer; exosomal transfer of circ_0006174 spreads DOX resistance from resistant CRC cells to sensitive CRC cells.
Molecular mechanism: Exosome-mediated intercellular transfer; ceRNA mechanism in which circ_0006174 sponges miR-1205 to upregulate CCND2, thereby promoting DOX resistance.
Biological pathway or process:

chemoresistance (promotes); drug resistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Transwell Assay; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model
Clinical significance:

Exosomal circ_0006174 can be used as a biomarker or diagnostic target for doxorubicin chemoresistance in colorectal cancer.
Description:

circ_0006174 is upregulated in DOX-resistant colorectal cancer tissues, cells, and exosomes derived from resistant CRC cells. Exosomal circ_0006174 promotes DOX chemoresistance and malignant cell behaviors by sponging miR-1205 and increasing CCND2 expression, while circ_0006174 knockdown enhances DOX sensitivity in vitro and in xenograft tumors. The study proposes exosomal circ_0006174 as a potential diagnostic biomarker or therapeutic target for DOX resistance in CRC.
Confidence score:

0.6521
Other information
Title:

Exosomal transfer of circ_0006174 contributes to the chemoresistance of doxorubicin in colorectal cancer by depending on the miR-1205/CCND2 axis.

Journal: Journal of physiology and biochemistry
Published: 2022
PubMed ID: 34792792
Study type:

combined biological and clinical study
Data availability: Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s13105-021-00831-y.
Code availability: -