circRNA basic information
circBase ID: hsa_circ_0096921
Name: hsa_circ_ANKS1B
Synonym: circAnks1b / hsa-circAnks1b
Host Gene: Anks1b
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 1060198
MONDO name: ischemic stroke
Disease details: ischemic stroke / IS
Disease DO ID:
 -
Disease MeSH ID:
 -
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

peri-infarct cortex; plasma
Cell lines:

HEK293T
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: Pak2, Ago2, IRE1alpha, XBP1
Associated microRNA: miR-130b-5p
Biological function: Promotes neurological recovery and neuronal survival after ischemic stroke rehabilitation; promotes protective ER stress; suppresses neuronal apoptosis.
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-130b-5p to disinhibit Pak2, enhancing IRE1alpha/XBP1 signaling and protective ER stress, thereby reducing apoptosis.
Biological pathway or process:

apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RIP (RNA Immunoprecipitation); FISH / smFISH; Luciferase Reporter Assay; Transfection; Western Blot; TUNEL; In Vivo Animal Model; ROC Analysis; Clinical Sample Validation
Clinical significance:

Plasma circAnks1b was higher in stroke patients receiving PE than sedentary patients and correlated with better neurological recovery; ROC analysis indicated high serum circAnks1b due to PE predicted better outcome (AUC=0.9352).
Description:

In tMCAO ischemic stroke models, physical exercise upregulates circAnks1b in peri-infarct cortex. circAnks1b sponges miR-130b-5p to relieve repression of Pak2, enhancing IRE1alpha/XBP1-associated protective ER stress and suppressing neuronal apoptosis, improving neurological recovery. Plasma circAnks1b increases with exercise in IS patients and is associated with better functional outcome.
Confidence score:

0.8484
Other information
Title:

Physical exercise-induced circAnks1b upregulation promotes protective endoplasmic reticulum stress and suppresses apoptosis via miR-130b-5p/Pak2 signaling in an ischemic stroke model.

Journal: CNS neuroscience & therapeutics
Published: 2024
PubMed ID: 39328024
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -