| Expression pattern: |
DN |
| Associated gene: |
Merlin |
| Associated microRNA: |
- |
| Biological function: |
sensitizes NSCLC cells to ferroptosis; promotes lipid peroxidation; suppresses proliferation, migration, invasion and tumor growth |
| Molecular mechanism: |
circPOLA2 binds Merlin and restricts Merlin phosphorylation at S518, activating Hippo signaling and inhibiting YAP to increase ferroptosis sensitivity and suppress tumorigenesis |
| Biological pathway or process: |
Hippo/YAP (inhibits); ferroptosis (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; ROC Analysis; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Western Blot; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
downregulated in NSCLC and correlated with advanced clinical stage; diagnostic value with AUC 0.879 |
| Description: |
circPOLA2 (hsa_circ_0004291) is down-regulated in NSCLC and acts as a tumor suppressor by promoting lipid peroxidation/ferroptosis and inhibiting proliferation and metastasis-related behaviors. Mechanistically, it binds Merlin, reduces Merlin Ser518 phosphorylation, activates Hippo signaling and suppresses YAP activity, increasing ferroptosis sensitivity and inhibiting tumor growth. |
| Confidence score: |
0.8832 |