| Expression pattern: |
UP |
| Associated gene: |
BUD13, KEAP1, EIF4A3, RANGAP1, SLTM |
| Associated microRNA: |
- |
| Biological function: |
circ_001653 knockdown mitigated SA-AKI by decreasing apoptosis, oxidative stress, and inflammation and improving proliferation in LPS-stimulated HK-2 cells and CLP-induced rat renal tissue. |
| Molecular mechanism: |
circ_001653 recruits the RBP BUD13, which promotes the stability of KEAP1 mRNA, thereby activating the KEAP1/Nrf2/HO-1 signaling pathway. |
| Biological pathway or process: |
NF-kappaB (other); apoptosis (other); proliferation (other); inflammation (inhibits); HIF-1 signaling (other); mitochondrial function (other); oxidative phosphorylation (other); immune regulation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transfection; TUNEL; EdU Staining; ELISA; Western Blot; RIP (RNA Immunoprecipitation); CCK8; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_001653 is up-regulated in SA-AKI models (LPS-stimulated HK-2 cells and CLP-induced rat kidney). Silencing circ_001653 protects against SA-AKI phenotypes by reducing apoptosis, oxidative stress and inflammation. Mechanistically, circ_001653 recruits the RBP BUD13 to stabilize KEAP1 mRNA and modulate the KEAP1/Nrf2/HO-1 signaling pathway. |
| Confidence score: |
0.7346 |