| Expression pattern: |
UP |
| Associated gene: |
NES |
| Associated microRNA: |
miR-31-5p |
| Biological function: |
promotes glioma cell proliferation, motility and invasion; promotes EMT; decreases radiosensitivity (promotes radioresistance); promotes tumor growth in vivo |
| Molecular mechanism: |
circATP8B4 functions as a ceRNA that sponges miR-31-5p, thereby relieving miR-31-5p-mediated repression of NES (post-transcriptional regulation). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); radioresistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; Wound Healing Assay; Transwell Assay; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
Targeting the ceRNA crosstalk may have potential to improve the outcome of glioma patients. |
| Description: |
circATP8B4 is up-regulated in human glioma and promotes malignant phenotypes (proliferation, motility, invasion/EMT) while reducing radiosensitivity. It acts as a ceRNA sponge for miR-31-5p to upregulate NES, and circATP8B4 knockdown suppresses xenograft growth and enhances radiotherapy efficacy. |
| Confidence score: |
0.8443 |