circRNA basic information
circBase ID: -
Name: hsa_circ_CCDC7
Synonym: -
Host Gene: CCDC7
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer / PCa
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent normal tissues / normal margins
Cell lines:

PC3; DU145; LNCaP; 293 T; HCT116; C4-2; PrEC LH; NCI-H660; LASCPC-01
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: FLRT3
Associated microRNA: -
Biological function: inhibits cell viability; suppresses migration and invasion; suppresses tumor growth in vivo; acts as a tumor suppressor
Molecular mechanism: protein-encoding circRNA; encodes a secretory protein (circCCDC7-180aa) that upregulates FLRT3
Biological pathway or process:

migration (inhibits); invasion (inhibits); proliferation (inhibits); metastasis (inhibits); EMT (inhibits)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; Western Blot; IF (Immunofluorescence); RIP (RNA Immunoprecipitation); Co-IP; ELISA; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Bioinformatics Analysis; Survival Analysis; ROC Analysis; Clinical Sample Validation
Clinical significance:

lower expression in tumor vs matched normal margins; lower in high Gleason score and metastasis; higher expression predicts better outcome; potential biomarker/prognostic marker; ROC AUC 0.703 for tumor vs normal in CPGEA
Description:

circCCDC7(15,16,17,18,19) is down-regulated in prostate cancer and lower in higher Gleason score and metastasis. Its higher expression is associated with better patient outcome. Functionally, circCCDC7 suppresses PCa cell viability, migration/invasion and xenograft growth mainly by encoding a secreted peptide (circCCDC7-180aa) that upregulates FLRT3 and mediates tumor-suppressive effects.
Confidence score:

0.8814
Other information
Title:

A protein-encoding CCDC7 circular RNA inhibits the progression of prostate cancer by up-regulating FLRT3.

Journal: NPJ precision oncology
Published: 2024
PubMed ID: 38225404
Study type:

combined biological and clinical study
Data availability: GSA-Human: HRA006338; GSE99857
Code availability: -