| Expression pattern: |
UP |
| Associated gene: |
AGO2, vimentin, VIM |
| Associated microRNA: |
miR-133a-5p |
| Biological function: |
circP4HB promotes EMT, vimentin expression, migration, invasion, xenograft lung tumor burden, and metastatic disease in NSCLC. |
| Molecular mechanism: |
circP4HB acts as a miR-133a-5p sponge, leading to upregulation of the miR-133a-5p target vimentin and promotion of EMT and metastasis. |
| Biological pathway or process: |
ceRNA regulation (promotes); EMT (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
B
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Northern Blot; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circP4HB expression is associated with lymph node metastasis and decreased patient survival in NSCLC. |
| Description: |
circP4HB is up-regulated in NSCLC tissues and cell lines and high expression is linked to lymph node metastasis and poorer survival. Functionally, it promotes NSCLC cell migration, invasion, EMT, and metastatic lung tumor burden by sponging miR-133a-5p and increasing vimentin expression. |
| Confidence score: |
0.8686 |