| Expression pattern: |
UP |
| Associated gene: |
VAPA, AGO2 |
| Associated microRNA: |
miR-421 |
| Biological function: |
Promotes prostate cancer cell proliferation and metastasis; inhibits apoptosis (knockdown increases apoptosis); promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge: circTP63 directly binds miR-421, thereby relieving miR-421-mediated repression of VAPA and increasing VAPA expression |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circTP63 could be a promising diagnostic and therapeutic target for PCa treatment. |
| Description: |
circTP63 is up-regulated in prostate cancer tissues/cell lines and mainly localizes to the cytoplasm. It promotes PCa proliferation and metastatic behaviors and inhibits apoptosis by sponging miR-421 to de-repress VAPA, enhancing tumor growth in a PC-3 xenograft model. |
| Confidence score: |
0.8568 |