| Expression pattern: |
UP |
| Associated gene: |
CCL5, Bax, Bcl-2 |
| Associated microRNA: |
miR-326 |
| Biological function: |
circABCB10 performs oncogenic functions in rectal cancer; circABCB10 knockdown promotes ferroptosis and apoptosis and inhibits tumor growth in vivo. |
| Molecular mechanism: |
circABCB10 acts as a sponge of miR-326 to positively regulate CCL5 expression, thereby suppressing ferroptosis and apoptosis in rectal cancer cells. |
| Biological pathway or process: |
ferroptosis (inhibits); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; In Vivo Animal Model; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
suggesting a potential therapeutic target for rectal cancer therapy |
| Description: |
circABCB10 is up-regulated in rectal cancer tissues and cell lines and functions as an oncogenic circRNA. Silencing circABCB10 promotes ferroptosis and apoptosis and suppresses xenograft tumor growth. Mechanistically, circABCB10 sponges miR-326 to positively regulate CCL5 expression, forming a circABCB10/miR-326/CCL5 regulatory axis. |
| Confidence score: |
0.7591 |