| Expression pattern: |
UP |
| Associated gene: |
BRD4, PTEN, PI3K, PKB |
| Associated microRNA: |
miR-488-3p |
| Biological function: |
Promotes NSCLC cell proliferation, invasion, migration, cell cycle progression and tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
circ_0000376 acts as a miR-488-3p sponge to regulate BRD4 expression and PTEN-mediated PI3K/PKB signaling pathway. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model |
| Clinical significance: |
Potential biomarker for NSCLC. |
| Description: |
circ_0000376 is up-regulated in NSCLC tissues and cell lines and functions as an oncogenic circRNA. Its knockdown suppresses proliferation, colony formation, invasion, migration, cell cycle progression and xenograft tumor growth while promoting apoptosis. Mechanistically, circ_0000376 sponges miR-488-3p to regulate BRD4 and modulates PTEN-mediated PI3K/PKB signaling. |
| Confidence score: |
0.7598 |