| Expression pattern: |
UP |
| Associated gene: |
METTL1, AGO2, PDK1, AKT |
| Associated microRNA: |
miR-147b-3p |
| Biological function: |
Promotes CRC cell proliferation, invasion and migration, inhibits apoptosis, and promotes lung metastasis in vivo. |
| Molecular mechanism: |
METTL1 installs m7G modification on circKDM1A (GG motif-dependent) to increase circKDM1A stability; cytoplasmic circKDM1A acts as a miRNA sponge for miR-147b-3p, upregulates PDK1, and activates the AKT pathway. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); m7A modification (other); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; FISH / smFISH; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis; MeRIP / MeRIP-seq; IHC (Immunohistochemistry); IF (Immunofluorescence); Clinical Sample Validation |
| Clinical significance: |
High expression of circKDM1A predicted poorer prognosis in CRC. |
| Description: |
In colorectal cancer, circKDM1A is up-regulated and promotes proliferation, migration/invasion, and metastasis while inhibiting apoptosis. METTL1-mediated m7G modification (GG motif-dependent) stabilizes circKDM1A, which sponges miR-147b-3p to upregulate PDK1 and activate the AKT pathway; high circKDM1A is associated with poor prognosis. |
| Confidence score: |
0.8941 |