| Expression pattern: |
UP |
| Associated gene: |
RBFOX2, AGO2 |
| Associated microRNA: |
miR-3174 |
| Biological function: |
promotes proliferation and migration/metastasis; inhibits apoptosis; promotes tumour growth in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-3174 to upregulate RBFOX2 (ceRNA mechanism). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Western Blot; IHC (Immunohistochemistry); Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
High expression of circ_0017065 was associated with the depth of tumour invasion, lymph node metastasis, tumor size and vascular invasion; potential biomarker/target. |
| Description: |
circRNA_0017065 is up-regulated in colorectal cancer tissues and promotes CRC cell proliferation and migration/metastasis while inhibiting apoptosis in vitro and tumour growth in vivo. It mainly localizes to the cytoplasm and promotes CRC progression by sponging miR-3174, thereby increasing RBFOX2 expression (circRNA_0017065/miR-3174/RBFOX2 axis). High circ_0017065 expression correlates with invasive clinicopathological features. |
| Confidence score: |
0.8635 |