| Expression pattern: |
UP |
| Associated gene: |
IL32, MMP9, TWF1, MS4A4A |
| Associated microRNA: |
Hsa-miR-409-3p, hsa-miR-579-5p, hsa-miR-1238-3p |
| Biological function: |
Upregulated in MDS patients with U2AF1 mutations; knockdown in U2AF1 Q157P K562 cells caused the most significant gene expression changes and altered IL32, MMP9, TWF1 and MS4A4A expression. |
| Molecular mechanism: |
Predicted miRNA interaction and downstream gene expression regulation after circRNA knockdown; possible involvement in U2AF1-mutation-associated circRNA biogenesis changes. |
| Biological pathway or process: |
ceRNA regulation (other); mRNA stability (other); other pathway/process (other) |
| Detected method: |
S
|
| Validation methods: |
RNA-seq; Clinical Sample Validation; Bioinformatics Analysis; Transfection; RT-qPCR |
| Clinical significance: |
Potential biomarker and therapeutic target investigation in U2AF1-mutated MDS. |
| Description: |
circCSNK1G3 was upregulated in U2AF1-mutated MDS and selected for functional study. Its knockdown caused substantial transcriptional changes in U2AF1 Q157P K562 cells, especially affecting IL32, MMP9, TWF1 and MS4A4A, supporting a biologically relevant role. |
| Confidence score: |
0.4997 |