| Expression pattern: |
UP |
| Associated gene: |
E2F7, Ago2 |
| Associated microRNA: |
miR-432-5p |
| Biological function: |
promotes proliferation, migration, invasion, stemness, and tumor growth in breast cancer |
| Molecular mechanism: |
acts as a miR-432-5p sponge to modulate E2F7 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); stemness (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Western Blot; IHC (Immunohistochemistry) |
| Clinical significance: |
High circFKBP8 expression was associated with worse overall survival in BC patients. |
| Description: |
circFKBP8 (hsa_circ_0000915) is upregulated in breast cancer tissues and cells and promotes malignant phenotypes (proliferation, migration, invasion, and stemness) and xenograft tumor growth. Mechanistically, it acts as a ceRNA by sponging miR-432-5p to increase E2F7 expression, and high circFKBP8 expression correlates with poor overall survival. |
| Confidence score: |
0.8489 |