| Expression pattern: |
DN |
| Associated gene: |
E-cadherin, N-cadherin, vimentin |
| Associated microRNA: |
miR-221 |
| Biological function: |
circMTO1 suppresses bladder cancer cell EMT, migration, invasion, metastasis, and tumor growth; low circMTO1 expression is associated with lymph node metastasis and poorer survival. |
| Molecular mechanism: |
circMTO1 acts as a sponge for miR-221 and regulates the E-cadherin/N-cadherin pathway to inhibit EMT and aggressiveness in bladder cancer cells. |
| Biological pathway or process: |
EMT (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; In Vivo Animal Model; Survival Analysis; Cohort Study |
| Clinical significance: |
lower circMTO1 levels were positively correlated with bladder cancer patients' metastasis and poorer survival; circMTO1 is a prognostic biomarker in bladder cancer. |
| Description: |
circMTO1 is down-regulated in bladder cancer tissues and cell lines, and low expression is associated with metastasis and poorer patient survival. Functionally, circMTO1 suppresses bladder cancer migration, invasion, EMT, metastasis, and xenograft tumor growth by sponging miR-221 and modulating EMT markers including E-cadherin, N-cadherin, and vimentin. |
| Confidence score: |
0.7811 |