circRNA basic information
circBase ID: hsa_circ_0072309
Name: hsa_circ_LIFR
Synonym: circLIFR / circ_0072309
Host Gene: LIFR
Genomic location(hg19): chr5:38523520-38530768:-
Genomic location(hg38): chr5:38523418-38530666:-
Subcellular localization: unclear
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary HCC tissue; paired adjacent non-tumorous liver tissue
Cell lines:

SK-Hep-1; HepG2; LO2; 293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: TBK1, p65, MMP13, MMP3, VEGF, MAPK
Associated microRNA: -
Biological function: Promotes HCC cell proliferation, migration and invasion; promotes cell cycle progression; reduces apoptosis.
Molecular mechanism: circLIFR interacts with TBK1 and counteracts TBK1 inhibitor effects, promoting NF-kappaB pathway activation and upregulating NF-kappaB downstream genes (MMP13, MMP3, VEGF, MAPK).
Biological pathway or process:

NF-kappaB (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Sanger Sequencing; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; FISH / smFISH; IF (Immunofluorescence); Transfection; CCK8; Wound Healing Assay; Transwell Assay; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

-
Description:

circLIFR (hsa_circ_0072309) is down-regulated in HCC tissues. Functional assays show circLIFR overexpression promotes HCC cell proliferation, migration/invasion, cell cycle progression and inhibits apoptosis, mechanistically by binding TBK1 and enhancing NF-kappaB activity with increased downstream gene expression (e.g., MMP13, MMP3, VEGF, MAPK).
Confidence score:

0.8072
Other information
Title:

Downregulation of circLIFR exerts cancer-promoting effects on hepatocellular carcinoma in vitro.

Journal: Frontiers in genetics
Published: 2022
PubMed ID: 36176304
Study type:

combined biological and clinical study
Data availability: https://www.frontiersin.org/articles/10.3389/fgene.2022.986322/full#supplementary-material
Code availability: -