| Expression pattern: |
UP |
| Associated gene: |
ZFX, MEK/ERK pathway, MEK1/2, ERK1/2 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
Facilitates CRC progression and tumorigenesis; promotes proliferation, angiogenesis, EMT, migration, cell cycle progression and in vivo tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
circ_0004585 functions as a ceRNA/miRNA sponge for miR-338-3p, regulates ZFX expression, and activates the MEK/ERK signaling pathway. |
| Biological pathway or process: |
ERK (promotes); proliferation (promotes); angiogenesis (promotes); EMT (promotes); apoptosis (inhibits); cell cycle (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Tube Formation Assay; Western Blot; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
might provide a potential therapeutic target for CRC; provided promising molecular targets for the diagnosis and treatment of CRC |
| Description: |
circ_0004585 is up-regulated in CRC tissues and cell lines and acts as an oncogenic circRNA. It promotes CRC proliferation, angiogenesis, EMT, migration and tumor growth while suppressing apoptosis, mainly by sponging miR-338-3p to regulate ZFX and activate the MEK/ERK pathway. The study suggests circ_0004585 may be a potential therapeutic target for CRC. |
| Confidence score: |
0.7899 |