| Expression pattern: |
UP |
| Associated gene: |
STAT3, Snail, IGF2BP2, METTL3, METTL14, Ago2 |
| Associated microRNA: |
miR-384 |
| Biological function: |
Promotes cell proliferation, migration, invasion and cell cycle progression; inhibits apoptosis; promotes EMT in ESCC cells. |
| Molecular mechanism: |
METTL3/METTL14-mediated m6A modification enhances circ_0006168 stability via IGF2BP2; circ_0006168 functions as a ceRNA sponge for miR-384 to upregulate STAT3 and activate STAT3/Snail-driven EMT. |
| Biological pathway or process: |
EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes); m6A modification (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
High expression of circ_0006168 was positively correlated with histological grade, TNM stage and lymphatic metastasis in ESCC. |
| Description: |
circ_0006168 is up-regulated in ESCC and acts as an oncogenic circRNA. Its m6A modification (mainly via METTL3) is recognized by IGF2BP2 to enhance circ_0006168 stability/expression, and circ_0006168 promotes proliferation and EMT-related migration/invasion by sponging miR-384 to increase STAT3 and downstream Snail. |
| Confidence score: |
0.8416 |