circRNA basic information
circBase ID: hsa_circ_0000228
Name: hsa_circ_ZEB1
Synonym: circZEB1
Host Gene: ZEB1
Genomic location(hg19): chr10:31661946-31676195:+
Genomic location(hg38): chr10:31373017-31387266:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

TNBC tissue; BC tissues
Cell lines:

MDA-MB-231; MDA-MB-436; ZR-75-1; MDA-MB-361; MCF-7; MCF-10 A; ZR-75-1; UACC812; MDA-MB-468; HCC-1937; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: OGT, YBX1, Ago2, FZD1, FGF10, NRP1, HES5
Associated microRNA: miR-337-3p
Biological function: promotes proliferation, migration, invasion, angiogenesis and metastasis in breast cancer/TNBC
Molecular mechanism: acts as a miRNA sponge for miR-337-3p to increase OGT, promoting O-GlcNAcylation and stabilizing YBX1, which activates transcription of multiple oncogenes (FZD1, FGF10, NRP1, HES5)
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Colony Formation Assay; CCK8; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; Co-IP; Western Blot; ChIP / ChIP-seq; Bioinformatics Analysis
Clinical significance:

-
Description:

circZEB1 (hsa_circ_0000228), derived from ZEB1, is up-regulated in breast cancer/TNBC and mainly localized in the cytoplasm. It promotes proliferation, migration/invasion, angiogenesis and metastasis by sponging miR-337-3p to upregulate OGT, enhancing O-GlcNAcylation and stabilizing YBX1, which then activates transcription of oncogenic targets such as FZD1, FGF10, NRP1 and HES5.
Confidence score:

0.8415
Other information
Title:

The circZEB1/miR-337-3p/OGT axis mediates angiogenesis and metastasis via O-GlcNAcylation and up-regulating YBX1 in breast cancer.

Journal: Heliyon
Published: 2024
PubMed ID: 39114035
Study type:

combined biological and clinical study
Data availability: GSE101124; GSE101122; https://doi.org/10.1016/j.heliyon.2024.e34079
Code availability: -