| Expression pattern: |
UP |
| Associated gene: |
COL1A1, PI3K/Akt pathway |
| Associated microRNA: |
miR-1184 |
| Biological function: |
Circ_0000523 promotes NPC cell proliferation, cell cycle progression, migration, invasion and lung metastasis. |
| Molecular mechanism: |
Circ_0000523 targets and negatively regulates miR-1184 to increase COL1A1 expression and activate the PI3K/Akt signaling pathway. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Circ_0000523 expression level was positively correlated with advanced TNM stage and may have potential in NPC diagnosis and treatment. |
| Description: |
Circ_0000523 is a METTL3-derived circRNA that is up-regulated in NPC tissues and cell lines. It promotes NPC progression by targeting and repressing miR-1184, thereby increasing COL1A1 expression and activating the PI3K/Akt pathway, leading to enhanced proliferation, cell cycle progression, migration, invasion and metastasis. |
| Confidence score: |
0.7662 |