| Expression pattern: |
UP |
| Associated gene: |
PDL1, AGO2, E-cadherin, N-cadherin, Ki67 |
| Associated microRNA: |
miR-1256 |
| Biological function: |
Circ_0007422 promotes CRC cell viability, proliferation, invasion, stemness, tumor growth and immune escape, and inhibits apoptosis and antitumor immunity. |
| Molecular mechanism: |
Circ_0007422 acts as a ceRNA for miR-1256 to increase PDL1 expression, thereby promoting CRC tumorigenesis and immune escape. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits); stemness (promotes); immune regulation (inhibits); EMT (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; ELISA; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0007422 expression was associated with poorer prognosis in CRC patients, and the circRNA had a high diagnostic value in CRC. |
| Description: |
Circ_0007422 is upregulated in colorectal cancer tissues and cell lines, and high expression is associated with poorer prognosis. Functionally, circ_0007422 promotes CRC proliferation, invasion, stemness, tumor growth and immune escape while suppressing apoptosis and antitumor immune activity. Mechanistically, it acts through a circ_0007422/miR-1256/PDL1 regulatory axis. |
| Confidence score: |
0.8596 |