circRNA basic information
circBase ID: hsa_circ_0001495
Name: hsa_circ_CCNB1
Synonym: circCCNB1
Host Gene: CCNB1
Genomic location(hg19): chr5:68470703-68471364:+
Genomic location(hg38): chr5:69174876-69175537:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

carcinoma tissues; para-carcinoma tissues
Cell lines:

HepG2; HCCLM3; HL-7702
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: GPM6A, DYNC1I1, AKT, ERK1/2
Associated microRNA: miR-106b-5p
Biological function: circCCNB1 silencing promotes proliferation, invasion, metastasis, cell cycle G1-S transition, colony formation, and xenograft tumor growth in HCC.
Molecular mechanism: ceRNA mechanism: circCCNB1 sponges miR-106b-5p to protect GPM6A; circCCNB1 silencing reduces GPM6A, increases DYNC1I1, and activates AKT/ERK phosphorylation to regulate cell cycle.
Biological pathway or process:

ceRNA regulation (other); cell cycle (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); PI3K/AKT (promotes); ERK (promotes); MAPK (promotes)
Detected method:
Q
M
Validation methods:

Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; FISH / smFISH; circRIP; Transfection; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; Western Blot; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

In HCC, circCCNB1 is down-regulated and functions as a miR-106b-5p sponge to maintain GPM6A expression. Silencing circCCNB1 lowers GPM6A, elevates DYNC1I1, activates AKT/ERK phosphorylation, and promotes cell-cycle progression, proliferation, invasion/metastasis, and xenograft tumor growth.
Confidence score:

0.7859
Other information
Title:

CircCCNB1 silencing acting as a miR-106b-5p sponge inhibited GPM6A expression to promote HCC progression by enhancing DYNC1I1 expression and activating the AKT/ERK signaling pathway.

Journal: International journal of biological sciences
Published: 2022
PubMed ID: 35002514
Study type:

combined biological and clinical study
Data availability: GSE97332; GSE108724; GSE101728
Code availability: -