circRNA basic information
circBase ID: -
Name: hsa_circ_CDR1
Synonym: CDR1as / CiRS-7
Host Gene: CDR1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

xenotransplanted tumour
Cell lines:

MCF-7; SKBR-3; MDA-MB-231; HCC-1937; MCF10; MCF-7/5-Fu; SKBR-3/5-Fu; MDA-MB-231/5-Fu; HCC-1937/5-Fu; MCF10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CCNE1
Associated microRNA: miR-7
Biological function: reduces 5-FU chemosensitivity (promotes chemoresistance), promotes colony formation/proliferation, inhibits apoptosis; promotes xenograft tumor growth
Molecular mechanism: CDR1as competitively binds (sponges) miR-7, thereby relieving miR-7-mediated repression of CCNE1 and decreasing 5-FU chemosensitivity.
Biological pathway or process:

chemoresistance (promotes); apoptosis (inhibits); proliferation (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; RNA Pull-Down; Luciferase Reporter Assay; In Vivo Animal Model
Clinical significance:

-
Description:

CDR1as is up-regulated in 5-FU-resistant breast cancer cells and promotes 5-FU chemoresistance. Mechanistically, CDR1as sponges miR-7, leading to increased CCNE1 expression, enhanced proliferation/colony formation, reduced apoptosis, and faster xenograft tumor growth under 5-FU treatment.
Confidence score:

0.5992
Other information
Title:

Inhibition of circular RNA CDR1as increases chemosensitivity of 5-FU-resistant BC cells through up-regulating miR-7.

Journal: Journal of cellular and molecular medicine
Published: 2019
PubMed ID: 30884120
Study type:

biological research
Data availability: -
Code availability: -