| Expression pattern: |
UP |
| Associated gene: |
EXT1-219aa, EXT1, PKM2, CD8+ T cells |
| Associated microRNA: |
- |
| Biological function: |
promotes gastric cancer progression, proliferation, metabolic reprogramming (glycolysis/pyruvate metabolism), and immune evasion; reduces CD8+ T cell number and function; decreases sensitivity to anti-PD-L1 therapy |
| Molecular mechanism: |
encodes EXT1-219aa that suppresses EXT1 phosphorylation/expression, leading to EXT1-dependent regulation of PKM2 glycosylation and nuclear localization; promotes glycolysis/pyruvate metabolism and immune escape via the EXT1/PKM2 axis |
| Biological pathway or process: |
immune regulation (inhibits); glycolysis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; Western Blot; In Vivo Animal Model; Flow Cytometry（Non-apoptosis/cycle）; RNA-seq |
| Clinical significance: |
Elevated serum hsa_circ_0008035 correlates with advanced tumor stages and lymph node metastasis; potential diagnostic biomarker/prognostic indicator |
| Description: |
hsa_circ_0008035 is up-regulated in gastric cancer patient serum and cell lines and is associated with advanced stage and lymph node metastasis. It promotes tumor growth and immune evasion by encoding EXT1-219aa to suppress EXT1, thereby enhancing PKM2 nuclear translocation/activity and glycolysis through an EXT1/PKM2 axis, reducing CD8+ T-cell infiltration/function and anti-PD-L1 sensitivity. |
| Confidence score: |
0.6745 |