| Expression pattern: |
UP |
| Associated gene: |
YBX1, GLO1 |
| Associated microRNA: |
- |
| Biological function: |
promotes cell viability and colony formation/cell growth; suppresses reactive oxygen species accumulation and apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
circMAN1A2_009 directly interacts with YBX1, enhances YBX1 Ser102 phosphorylation and nuclear localization, and activates GLO1 transcription/expression to drive CADC cell growth and reduce ROS/apoptosis |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); p53 signaling (other); mitochondrial function (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; RIP (RNA Immunoprecipitation); IF (Immunofluorescence); Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); TUNEL; ROC Analysis |
| Clinical significance: |
Upregulated in CADC tissues and shows diagnostic value with AUC 0.8075 for discriminating CADC from normal cervical tissue |
| Description: |
circMAN1A2_009 is up-regulated in cervical adenocarcinoma and promotes CADC cell growth and tumorigenesis while suppressing ROS accumulation and apoptosis. Mechanistically, it binds YBX1 to enhance p-YBX1S102 and YBX1 nuclear localization, thereby activating GLO1 expression; it also shows diagnostic potential (AUC 0.8075). |
| Confidence score: |
0.8933 |