circRNA basic information
circBase ID: hsa_circ_0069718
Name: hsa_circ_DCUN1D4
Synonym: circ_0069718
Host Gene: DCUN1D4
Genomic location(hg19): chr4:52729602-52765544:+
Genomic location(hg38): chr4:51863436-51899378:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

Tumor tissues; corresponding para-cancerous tissues; adjacent non-tumor tissues
Cell lines:

MCF-10AT; MCF-7; MDA-MB-231; MDA-MB-468; MCF-10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: NFIB, DCUN1D4, Bax, Bcl-2, ki-67, E-cadherin
Associated microRNA: miR-590-5p
Biological function: Circ_0069718 acts as an oncogene in breast cancer, promoting cell proliferation, colony formation, DNA synthesis, migration, invasion, and tumor growth, while inhibiting apoptosis.
Molecular mechanism: Circ_0069718 functions as a ceRNA/sponge for miR-590-5p, thereby up-regulating NFIB expression and promoting breast cancer progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes)
Detected method:
Q
Validation methods:

Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

Circ_0069718 may be a potential therapeutic strategy or target in breast cancer.
Description:

Circ_0069718 is up-regulated in breast cancer tissues and cell lines and acts as an oncogenic circRNA. It promotes malignant phenotypes and tumor growth by sponging miR-590-5p and thereby increasing NFIB expression, suggesting potential therapeutic relevance in breast cancer.
Confidence score:

0.8439
Other information
Title:

Circ_0069718 promotes the progression of breast cancer by up-regulating NFIB through sequestering miR-590-5p.

Journal: Mammalian genome : official journal of the International Mammalian Genome Society
Published: 2021
PubMed ID: 34632534
Study type:

combined biological and clinical study
Data availability: The analyzed datasets generated during the study are available from the corresponding author on reasonable request.
Code availability: -