| Expression pattern: |
UP |
| Associated gene: |
IGF2BP2, HuR, CDCA4 |
| Associated microRNA: |
- |
| Biological function: |
facilitates breast cancer cell proliferation |
| Molecular mechanism: |
IGF2BP2 binds circRNF20 to prevent its degradation and promote circRNF20 binding to HuR, which increases CDCA4 expression. |
| Biological pathway or process: |
proliferation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RIP (RNA Immunoprecipitation); RNA Pull-Down; Actinomycin D / DRB Stability Assay; FISH / smFISH; IF (Immunofluorescence); Transfection; CCK8; Colony Formation Assay; EdU Staining; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circRNF20 is up-regulated in breast cancer cell lines and promotes cancer cell proliferation. Mechanistically, IGF2BP2 stabilizes circRNF20, enabling circRNF20 to bind HuR and enhance CDCA4 expression, forming an IGF2BP2-circRNF20-HuR-CDCA4 pro-proliferative axis. |
| Confidence score: |
0.7799 |