| Expression pattern: |
DN |
| Associated gene: |
PPP1R3C, QKI, mTOR, Myc, AGO2 |
| Associated microRNA: |
miR-1203 |
| Biological function: |
inhibits CRC cell proliferation, migration and invasion; promotes apoptosis; inhibits rapamycin resistance |
| Molecular mechanism: |
QKI promotes circ_0001766 biogenesis from PDIA4 pre-mRNA; circ_0001766 acts as a ceRNA sponging miR-1203 to stabilize PPP1R3C mRNA, thereby inhibiting mTOR signaling and Myc phosphorylation and reducing rapamycin resistance |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); metastasis (inhibits); mTOR/Myc pathway (inhibits); drug resistance (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Higher circ_0001766 expression correlates with better prognosis (OS and PFS) and is associated with metastasis status |
| Description: |
circ_0001766 (hsa_circ_0001766), generated from PDIA4 and promoted by the RBP QKI, is down-regulated in colorectal cancer. It localizes mainly in the cytoplasm and suppresses CRC proliferation, migration/invasion, metastasis and rapamycin resistance by sponging miR-1203 to sustain PPP1R3C expression, thereby inhibiting mTOR signaling and Myc phosphorylation. Higher circ_0001766 expression is associated with better OS/PFS. |
| Confidence score: |
0.8684 |