| Expression pattern: |
UP |
| Associated gene: |
XBP1 |
| Associated microRNA: |
miR-516b-5p |
| Biological function: |
promotes migration, invasion and proliferation of ESCC cells |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-516b-5p to up-regulate XBP1 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Transfection; Wound Healing Assay; Transwell Assay; MTT; Colony Formation Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
High expression of circ_0006168 in tumor tissues was correlated with large tumor size, positive lymph node metastasis and advanced TNM stage in ESCC patients. |
| Description: |
Circ_0006168 is up-regulated in ESCC tissues and cell lines and promotes ESCC cell proliferation, migration, and invasion. Mechanistically, it functions as a ceRNA by sponging miR-516b-5p, thereby increasing XBP1 expression; rescue experiments support the circ_0006168/miR-516b-5p/XBP1 axis. |
| Confidence score: |
0.778 |