| Expression pattern: |
UP |
| Associated gene: |
CCNT2, AGO2 |
| Associated microRNA: |
miR-130a-5p |
| Biological function: |
promotes HCC cell proliferation and tumor growth; increases G2/M phase cells |
| Molecular mechanism: |
circROBO1 acts as a competing endogenous RNA (miRNA sponge) for miR-130a-5p, relieving repression on CCNT2 and increasing CCNT2 expression |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; In Vivo Animal Model; H&E Staining; Survival Analysis; Clinical Sample Validation; Cohort Study |
| Clinical significance: |
elevated circROBO1 was closely correlated with worse prognosis for HCC patients; HCC cases with higher circROBO1 had worse OS and RFS |
| Description: |
circROBO1 (hsa_circ_0066568), derived from ROBO1, is upregulated in HCC and mainly localized in the cytoplasm. It promotes HCC proliferation and tumor growth by sponging miR-130a-5p, thereby upregulating CCNT2, and high circROBO1 predicts worse OS/RFS. |
| Confidence score: |
0.8768 |