| Expression pattern: |
UP |
| Associated gene: |
TLR4 |
| Associated microRNA: |
miR-527 |
| Biological function: |
promotes LPS-induced PDLC injury (apoptosis, inflammation, oxidative stress) and inhibits viability/proliferation |
| Molecular mechanism: |
ceRNA mechanism: circ_0087199 sponges miR-527 to regulate TLR4 |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits); inflammation (promotes); oxidative phosphorylation (unclear); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_0087199 is up-regulated in periodontal ligament tissues from periodontitis patients and in LPS-stimulated PDLCs. Functionally, circ_0087199 promotes LPS-induced PDLC injury (reduced viability/proliferation and increased apoptosis, inflammation, and oxidative stress) by acting as a ceRNA that sponges miR-527 to increase TLR4. |
| Confidence score: |
0.7416 |