| Expression pattern: |
UP |
| Associated gene: |
GLS |
| Associated microRNA: |
miR-375 |
| Biological function: |
Promotes glioma cell proliferation, migration, invasion, tumor growth and glutamine catabolism, and inhibits apoptosis. |
| Molecular mechanism: |
circ-CREBBP functions as a miRNA sponge for miR-375 and indirectly up-regulates GLS expression through the circ-CREBBP/miR-375/GLS axis. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glutaminolysis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot |
| Clinical significance: |
High circ-CREBBP expression was correlated with WHO grade stage and predicted shorter overall survival in glioma patients. |
| Description: |
circ-CREBBP is up-regulated in glioma tissues and cells, and its high expression is associated with poor overall survival. Functionally, circ-CREBBP promotes glioma proliferation, migration, invasion, tumor growth and glutamine catabolism while suppressing apoptosis. Mechanistically, it sponges miR-375 to relieve repression of GLS, forming a circ-CREBBP/miR-375/GLS axis. |
| Confidence score: |
0.8353 |