circRNA basic information
circBase ID: -
Name: hsa_circ_FARSA
Synonym: -
Host Gene: FARSA
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer / BCa
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissues; normal paracancerous tissue samples; normal bladder tissues
Cell lines:

T24; UM-UC-3; 5637; J82
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MAPK/ERK, SIRT6, ITGA5, PAK1, MUC1, NOB1
Associated microRNA: miR-330-5p / miR-330
Biological function: Promotes proliferation, invasion and migration, and inhibits apoptosis of bladder cancer cells; promotes tumor growth in vivo.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-330, thereby modulating downstream oncogene expression (MAPK/ERK, SIRT6, ITGA5, PAK1, MUC1, NOB1).
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (promotes); MAPK/ERK (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model
Clinical significance:

High circFARSA expression is associated with more advanced tumor stage, larger tumor size, higher grade, and lymph node metastasis in bladder cancer patients.
Description:

circFARSA is up-regulated in bladder cancer tissues and cell lines and promotes malignant phenotypes (proliferation, migration/invasion) while inhibiting apoptosis. Mechanistically, it acts as a ceRNA by sponging miR-330-5p, thereby increasing downstream oncogenic proteins such as MAPK/ERK, SIRT6, ITGA5, PAK1, MUC1 and NOB1, and its knockdown suppresses tumor growth in a T24 xenograft model.
Confidence score:

0.6837
Other information
Title:

The circular RNA circFARSA sponges microRNA-330-5p in tumor cells with bladder cancer phenotype.

Journal: BMC cancer
Published: 2022
PubMed ID: 35395756
Study type:

combined biological and clinical study
Data availability: Supplementary material available at 10.1186/s12885-022-09467-7
Code availability: -