| Expression pattern: |
UP |
| Associated gene: |
UBR5 mRNA, UBR5, Ago2, RISC |
| Associated microRNA: |
miR-1179 |
| Biological function: |
Promotes malignant growth, proliferation, viability, invasion, migration, xenograft tumor growth, and lung metastasis of TNBC cells; high expression is associated with shorter overall survival. |
| Molecular mechanism: |
circUBR5 acts as a ceRNA/miRNA sponge for miR-1179, reducing miR-1179-mediated targeting of UBR5 mRNA and thereby up-regulating UBR5 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Higher circUBR5 expression in TNBC tissues was associated with shorter overall survival and may serve as a prognostic biomarker and candidate therapeutic target. |
| Description: |
circUBR5 is a UBR5-derived human circRNA that is up-regulated in TNBC tissues and cells and whose high expression predicts poorer overall survival. It promotes TNBC cell proliferation, viability, migration, invasion, xenograft growth, and lung metastasis by sponging miR-1179 and up-regulating UBR5. The study supports circUBR5 as a prognostic biomarker and potential therapeutic target in TNBC. |
| Confidence score: |
0.8658 |