| Expression pattern: |
UP |
| Associated gene: |
HuR, XIRP1 |
| Associated microRNA: |
- |
| Biological function: |
promotes invasion and migration; promotes tumor aggressiveness/expansion; promotes EMT |
| Molecular mechanism: |
HIF1alpha induces circPLOD2a; circPLOD2a binds HuR and competitively inhibits HuR-XIRP1 mRNA interaction, causing XIRP1 mRNA degradation/downregulation |
| Biological pathway or process: |
migration (promotes); invasion (promotes); EMT (promotes); mRNA stability (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; divergent primers PCR; Sanger Sequencing; RNA Pull-Down; Western Blot; RIP (RNA Immunoprecipitation); Transfection; Transwell Assay; Wound Healing Assay; Actinomycin D / DRB Stability Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circPLOD2a is highly expressed in GBM compared with lower grade glioma and is negatively correlated with XIRP1 expression |
| Description: |
circPLOD2a is up-regulated by hypoxia/HIF1alpha in glioblastoma and promotes invasion/migration and xenograft progression. It binds the RBP HuR and competitively disrupts HuR binding to XIRP1 mRNA, reducing XIRP1 stability/expression and enhancing EMT-associated aggressiveness. |
| Confidence score: |
0.8615 |