| Expression pattern: |
UP |
| Associated gene: |
MYO5A |
| Associated microRNA: |
miR-370-3p, miR-136-5p |
| Biological function: |
Promotes ESCC cell proliferation and inhibits apoptosis; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-370-3p/miR-136-5p to relieve repression of MYO5A, promoting ESCC progression. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Actinomycin D / DRB Stability Assay; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; In Vivo Animal Model; ROC Analysis |
| Clinical significance: |
circFNDC3B had high diagnostic potential (AUC 0.8586) and was proposed as a diagnostic marker for ESCC. |
| Description: |
circFNDC3B is up-regulated in ESCC tissues and cell lines and promotes proliferation while inhibiting apoptosis. Mechanistically, it functions as a ceRNA sponge for miR-370-3p and miR-136-5p to increase MYO5A expression, enhancing ESCC progression and tumor growth in xenografts; it also shows diagnostic potential by ROC analysis. |
| Confidence score: |
0.8094 |