circRNA basic information
circBase ID: hsa_circ_0010220
Name: -
Synonym: circRNA-0010220
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

osteosarcoma tissues; adjacent normal tissues
Cell lines:

MG-63; HOS-2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: EZH2, CTNNBIP1, beta-catenin
Associated microRNA: -
Biological function: promotes proliferation; promotes migration; promotes invasion; promotes metastasis
Molecular mechanism: circRNA-0010220 recruits EZH2 to the CTNNBIP1 promoter to promote H3K27me3-mediated epigenetic silencing of CTNNBIP1, activating Wnt/beta-catenin signaling.
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; RNA Pull-Down; RIP (RNA Immunoprecipitation); FISH / smFISH; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis
Clinical significance:

-
Description:

In osteosarcoma, BMSC-derived EVs deliver circRNA-0010220 into OS cells, where it localizes to the nucleus and binds EZH2. This promotes EZH2-mediated H3K27me3 at the CTNNBIP1 promoter, suppressing CTNNBIP1 and activating Wnt/beta-catenin signaling, thereby enhancing proliferation, migration/invasion, and metastasis.
Confidence score:

0.8416
Other information
Title:

BMSC-derived extracellular vesicles enhance osteosarcoma proliferation and metastasis via the circRNA-0010220/beta-catenin pathway.

Journal: Cell death & disease
Published: 2026
PubMed ID: 41876483
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available on request from the corresponding author.
Code availability: -