circRNA basic information
circBase ID: -
Name: hsa_circ_CEP128
Synonym: circ-CEP128
Host Gene: CEP128
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer / BCa
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

bladder tumor tissues; paracarcinoma tissues; human bladder tissue specimens; blood samples; tumor tissues
Cell lines:

SV-HUC-1; 293T; J82; T24
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MYD88, p38, ERK, JNK, p-p38, p-ERK, p-JNK
Associated microRNA: miR-145-5p
Biological function: Promotes bladder cancer progression, cell viability, proliferation, migration/mobility, cell cycle progression and tumor growth, while inhibiting apoptosis.
Molecular mechanism: circCEP128 acts as a sponge for miR-145-5p, thereby modulating MYD88 and downstream MAPK signaling pathway proteins.
Biological pathway or process:

MAPK (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; BrdU; Wound Healing Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

circCEP128 was overexpressed in higher tumor differentiation stage, III-IV T stage, and N2-N3 lymph node metastasis stage of bladder cancer, suggesting association with advanced clinicopathologic features.
Description:

circCEP128 is upregulated in bladder cancer tissues, blood, and bladder cancer cells. It promotes bladder cancer progression by sponging miR-145-5p, increasing MYD88 and activating downstream MAPK signaling proteins; its knockdown suppresses proliferation, migration, cell cycle progression and xenograft tumor growth while increasing apoptosis.
Confidence score:

0.8325
Other information
Title:

Circular RNA CEP128 promotes bladder cancer progression by regulating Mir-145-5p/Myd88 via MAPK signaling pathway.

Journal: International journal of cancer
Published: 2019
PubMed ID: 30939216
Study type:

combined biological and clinical study
Data availability: GSE92675; GSE40355; Supporting Information Table S1; Supporting Information Table S2; Supporting Information Table S3; Supporting Information Table S4
Code availability: -