| Expression pattern: |
DN |
| Associated gene: |
TIMP2, Ago2 |
| Associated microRNA: |
miR-429 |
| Biological function: |
Inhibits PTC cell proliferation and migration and suppresses tumor growth in vivo (tumor-suppressive role). |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-429 to upregulate TIMP2; validated by luciferase, RIP (Ago2), and FISH co-localization; rescue experiments support the axis. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); ceRNA regulation (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
Potential biomarker and therapeutic target for PTC (no explicit prognosis/diagnosis performance reported). |
| Description: |
circLIFR (hsa_circ_0072309), derived from LIFR, is down-regulated in papillary thyroid cancer and acts as a tumor suppressor by inhibiting proliferation and migration. It functions as a miR-429 sponge to relieve repression of TIMP2, and circLIFR overexpression suppresses xenograft tumor growth. |
| Confidence score: |
0.866 |