circRNA basic information
circBase ID: hsa_circ_0043278
Name: hsa_circ_TADA2A
Synonym: circ_0043278 / hsa_102049
Host Gene: TADA2A
Genomic location(hg19): chr17:35797838-35800763:+
Genomic location(hg38): chr17:37437737-37440662:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; corresponding normal tissues; lung tumor samples; control samples; metastasis tissues; non-metastasis tissues
Cell lines:

16HBE; H1975; A549; SPC-A1; H1299
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ROCK1, CDKN1B, AKT3
Associated microRNA: miR-520f, miR-1299, miR-203, miR-450b-3p, miR-526b
Biological function: Promotes NSCLC cell proliferation, viability, tumor sphere formation, invasion, migration, and in vivo tumor growth.
Molecular mechanism: circ_0043278 functions as a ceRNA by sponging miR-520f, thereby increasing ROCK1, CDKN1B, and AKT3 expression in NSCLC cells.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); migration (promotes); metastasis (promotes); stemness (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Luciferase Reporter Assay; RNA Pull-Down
Clinical significance:

circ_0043278 may serve as a potential diagnostic and prognostic biomarker for NSCLC; increased expression was correlated with differentiation grade, distal metastasis, and TNM stage.
Description:

hsa_circ_0043278/circ_0043278 is up-regulated in NSCLC tissues and cell lines and is associated with metastasis-related clinicopathologic features. Functionally, it promotes NSCLC proliferation, invasion, migration, tumor sphere formation, and xenograft tumor growth. Mechanistically, circ_0043278 sponges miR-520f to increase ROCK1, CDKN1B, and AKT3 expression, suggesting potential biomarker and therapeutic relevance.
Confidence score:

0.7295
Other information
Title:

A novel circular RNA, hsa_circ_0043278, acts as a potential biomarker and promotes non-small cell lung cancer cell proliferation and migration by regulating miR-520f.

Journal: Artificial cells, nanomedicine, and biotechnology
Published: 2019
PubMed ID: 30873868
Study type:

combined biological and clinical study
Data availability: GSE101586; GEO, http://www.ncbi.nlm.nih.gov/geo; Supplementary Excel 1
Code availability: -