| Expression pattern: |
UP |
| Associated gene: |
IL-10, STAT3 |
| Associated microRNA: |
miR-379-3 p |
| Biological function: |
promotes macrophage M2 polarization and promotes CRC metastatic nodule formation (metastasis) via exosomal transfer |
| Molecular mechanism: |
Exosomal circPOLQ enters macrophages and sponges miR-379-3p to de-repress IL-10, activating STAT3 signaling and driving M2 polarization. |
| Biological pathway or process: |
JAK/STAT (promotes); metastasis (promotes); migration (promotes); invasion (promotes); EMT (promotes); immune regulation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; ISH (In Situ Hybridization); Clinical Sample Validation; Survival Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Western Blot; CCK8; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
Higher circPOLQ expression was associated with poorer prognosis in patients with CRC. |
| Description: |
circPOLQ is up-regulated in colorectal cancer tissues and enriched in CRC cell-derived exosomes. After being transferred into macrophages, circPOLQ sponges miR-379-3p to increase IL-10 and activate STAT3 signaling, promoting M2 polarization and enhancing CRC invasion/metastatic nodule formation. High circPOLQ expression correlates with poorer overall survival in CRC patients. |
| Confidence score: |
0.8693 |