| Expression pattern: |
UP |
| Associated gene: |
CDK4 |
| Associated microRNA: |
miR-486 |
| Biological function: |
hsa_circ_0016788 acts as an oncogenic circRNA in HCC; silencing hsa_circ_0016788 inhibits HCC cell proliferation and invasion, promotes apoptosis, and suppresses tumor growth in vivo. |
| Molecular mechanism: |
hsa_circ_0016788 functions through a miR-486/CDK4 regulatory pathway; miR-486 targets hsa_circ_0016788 and CDK4, and hsa_circ_0016788 silencing increases miR-486 expression and decreases CDK4 protein expression. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0016788 had high diagnostic efficiency for HCC patients with AUC 0.851 and acted as a biomarker for HCC detection. |
| Description: |
hsa_circ_0016788 is up-regulated in HCC tissues and cell lines and functions as an oncogenic circRNA. Its knockdown suppresses proliferation, invasion, and xenograft tumor growth while promoting apoptosis, likely through the miR-486/CDK4 pathway. The study also suggests diagnostic potential for hsa_circ_0016788 in HCC, with ROC AUC of 0.851. |
| Confidence score: |
0.817 |